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IEEE TRANSACTIONS ON PLASMA SCIENCE, VOL. 32, NO. 4, AUGUST 2004
TABLE II
COMPARISON BETWEEN FIELD MAGNITUDES PREDICTED BY THE XFDTD MODEL AND
THOSE CALCULATED FROM FORWARD (F) AND REFLECTED (R) POWERS AT 1 GHz
and BSS saturated glass fiber filter), measurements were per-
not presently available in our laboratory. A comparison of the
field and
at their respective maxima
formed using an open-ended dielectric probe kit13 in conjunction
magnitudes of the
in the standing wave pattern in the waveguide at a frequency
with an automated vector network analyzer (HP model 8720B).
of 1 GHz showed good agreement for forward powers in the
In making these measurements and applying the results to the
range 0.5­5 W (Table II). The fact that the computations based
XFDTD model, a number of issues needed to be addressed to
on waveguide theory utilized values of power measured by
achieve accurate reliable results [17].
a wattmeter having a manufacturer-specified accuracy of 5%
One issue was the minimum thickness of material required
could have contributed to differences between the two methods
to obtain accurate dielectric property measurements. In the case
(maximum difference 8.5%). Moreover, XFDTD simulations
of materials with high water content, the open-ended coaxial
took into account field disturbances from the probe launch and
probe technique provides accurate dielectric properties with a
waveguide slots, whereas computations based on waveguide
minimum sample thickness of 5­6 mm in the frequency range
theory did not.
of interest [18], [19]. Other dielectrics typically require a greater
Surface plots of the  field and  computed at a frequency
thickness that depends on the value of the dielectric constant of
of 1 GHz and a forward power of 0.5 W are presented in Fig. 6.
the material. For the BSS-saturated glass fiber filters it was es-
plane of the waveguide
The results are plotted in the central
tablished that a sample thickness equivalent to that of 20 stacked
at the location where the glass fiber filter would be placed in
filters, i.e., an approximate thickness of 5.2 mm was sufficient
experiments. Here the  axis is along the longer cross-sectional
to give accurately measured dielectric data. In the case of the
dimension, the axis is along the shorter cross-sectional dimen-
dental putty and Plexiglas, a thickness of 16 mm was sufficient.
sion, and the axis is along the length of the waveguide (Fig. 4).
The permittivity of BSS and a BSS saturated glass fiber filter
It is observed that the distances from the shorting plate of the two
changed with frequency as expected for a first-order Drude ma-
existing  field maxima are 16.31 and 35.20 cm, respectively.
terial [11]. The Plexiglas and dental putty permittivities did not
This provides an estimated waveguide wavelength of 37.8 cm,
change appreciably within the frequency range of interest.
which is the same as the theoretical value. The  maximum oc-
The second issue was whether the presence of the chromaffin
curred at a quarter waveguide wavelength from the  maximum
cells on the glass fiber filter appreciably affected the dielectric
as expected from theory. In addition, the magnitudes of  and
properties of the filter. To determine this, dielectric property
at the maxima were computed by XFDTD to be 172.3 V/m
measurements were made on a suspension of 8 million chro-
and 4.49 T, respectively. These values agree well with the values
maffin cells in 20 ml of BSS, the maximum number of cells
172.2 V/m and 4.57 T predicted by waveguide theory.
that are used in experiments. A difference of less than 2% was
The overall field patterns computed by XFDTD [Fig. 6(b)]
measured in the dielectric properties of BSS with cells and BSS
and predicted by waveguide theory based on measurements of
alone, which is in agreement with published results [20], [21].
forward and reflected power [Fig. 6(a)] agree closely. Although
This suggests that the presence of the cells can be ignored when
the XFDTD model takes into account the near field of the probe
modeling the glass fiber filter.
launch, which rapidly falls off with distance from the probe, this
does not appear to affect the overall standing wave pattern in
III. RESULTS AND DISCUSSION
the waveguide. Also, even though the overall  patterns were
A. Model Validation
in agreement, the XFDTD plots revealed small features not pre-
To test the accuracy of the XFDTD model, the numerically
dicted by theory. For example, small ripples in the second max-
computed  field and  within the waveguide, in the absence
imum of the  component of  were localized in a region of
of the cell perfusion apparatus, were compared with those
the subgrid in the vicinity of the top and bottom slots in the
computed from well-known waveguide theory [22] based
waveguide walls. These features disappeared when the top and
on measured values of forward and reflected power. Actual
bottom slots were not implemented in the model. Also, in the
fields and
in the waveguide were not
measurements of
region of the same standing wave maximum, there were small
performed because nonperturbing field measuring probes are
local maxima and minima in the  component of  along the
subgrid boundaries in the  direction. These variations existed
over a width of a single Yee cell (0.3048 cm) along the  axis
and ceased to exist outside the subgrid. The variations remained
13Hewlett
Packard (HP) model 85 070A, Palo Alto, CA